Part:BBa_J100233
gapA promoter - predicted constitutive on
We found the discussion of the gapA gene in the lab report, "A Constitutive Expression System for Cellulase Secretion in Escherichia coli and Its Use in Bioethanol Production." The lab determined which gene promoter would express cellulase the most efficiently. The gapA promoter worked well because it was constitutive. In looking for the gene promoter sequence of gapA, we came across another article entitled "The Escherichiacoli gapA Gene Is Transcribed by the Vegetative RNA Polymerase Holoenzyme Eur70 and by the Heat Shock RNA Polymerase Eu32. This article gave us the sequence of gapA gene promoter and included all of the -10 sequences and -35 sequences for the gapA gene. Our oligos is only a portion of the gene promoter
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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